Quantitative Polymerase Chain Reaction (qPCR) is a powerful molecular biology technique used to quantify the amount of a specific DNA sequence in a sample. It builds upon the principles of traditional PCR but incorporates fluorescent dyes or probes to measure DNA amplification in real time.
qPCR technology typically involves the following procedure:
- Denaturation:
- The thermocycler heats up to roughly 95 °C.
- The double-stranded DNA separates into single strands.
- Annealing:
- The temperature lowers down to 45-65 °C.
- The single-stranded primers attach to the appropriate ends of the target sequence.
- Extension:
- The temperature is raised to around 68-72°C.
- DNA polymerase synthesizes a new DNA strand using the primers as templates.
- Cycling:
- The thermal cycles are repeated multiple times (usually 20-40 cycles).
- Each cycle doubles the amount of DNA.
- Quantification:
- The qPCR instrument with high-sensitivity spectrometers records the fluorescence emitted at the end of each amplification cycle.
Our qPCR diagnostic products cover a wide range of applications listed in the test menu below.
The qPCR reagents are designed to be used with our special qPCR Instrument. Our qPCR instrument is designed for small-scale testing in research labs and point-of-care testing (POCT), delivering fast and accurate DNA analysis.
Test Menu
| Cat. No. | Product Name | Detection of | Package |
| WI6200 | Cellex qSARS-CoV-2/Flu A/Flu B PCR Assay | Flu A, Flu B, and Covid-19 virus | 48 T/K |
| WI6201 | Cellex qRSV/ADV/hMPV PCR Assay | RSV, Adnovirus, and Human metapneumoviruses | 48 T/K |
| WI6202 | Cellex qHPIV/RV/EV PCR Assay | Human parainfluenza virus, rhinovirus, and enterovirus | 48 T/K |
| WI6203 | Cellex qCoV PCR Assays | Human common cold Cov 229E, NL63, OC43, and HKU1 | 48 T/K |
| WI6204 | Cellex qSTD Triple PCR Assays | Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis | 48 T/K |
| WI6205 | Cellex qMP PCR Tripple Assays | Mycoplasma genitalium/Mycoplasma hominis/Human ureaplasma (U. urealyticum & U. parvum) | 48 T/K |
| WI6206 | Cellex qGBS PCR Assay | Group B streptococcus | 48 T/K |
| WI6207 | Cellex qY MD PCR Test | Y chromosome microdeletions associated with male infertility | 48 T/K |
| WI6208 | Cellex qMTHFR C677T PCR Assay | Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, associated with various diseases (vascular, cancers, neurology, diabetes, psoriasis, etc.) | 48 T/K |
| WI6209 | Cellex qHPV 24 Dx Assay | Detection of all 24 HPV types | 48 T/K |
| WI6210 | Cellex qHPV 12 Dx Assay | Detection and grouping of 12 High-Risk HPV types including 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | 48 T/K |
| WI6211 | Cellex qHPV 11 Dx Assay | Detection and grouping of 11 High-Risk HPV types including 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68 | 48 T/K |
| WI6212 | Cellex qCC Screening Assay | Screening of colon cancer | 48 T/K |
| WI6213 | Cellex qDEN Multiplex PCR Assay | Dengue virus types 1-4; Detect and differentiate Types 1 and 2; detect Types 3 and 4 as a group | 48 T/K |
| WI6214 | Cellex qAPOE4 PCR Assay | Detection and differentiation of heterozygous and homozygous of APOE4 mutation indicative of Alzheimer’s risk | 48 T/K |
| WI6215 | Cellex qSD PCR Assay | Sickle cell disease | 48 T/K |
| WI6220 | Cellex qEGFR Multiplex PCR Assay | EGFR gene mutations of Ex19Del, L858R, T790M, which cause non-small-cell lung cancer | 48 T/K |
| WI6221 | Cellex qBRAF V600E PCR Assay | Thyroid cancer caused by BRAF V600E mutation | 48 T/K |
| WI6222 | Cellex qALK/ROS1/MET PCR Assay | ALK/ROS1/MET gene fusions and mutations, which cause lung adenocarcinomas | 48 T/K |
| WI6223 | Cellex qKRAS/BRAF/NRAS/PIK3CA PCR Assay | Mutations in KRAS/BRAF/NRAS/PIK3CA genes, which cause colorectal cancer | 48 T/K |
| WI6224 | Cellex qHER2 PCR Assay | HER2 mutation, which causes breast cancer | 48 T/K |
| WI6225 | Cellex qJAK2 V617F PCR Assay | JAK2 V617F mutation, which causes myeloproliferative cancer | 48 T/K |