qPCR Test Overview

qPCR Test Overview


Quantitative Polymerase Chain Reaction (qPCR) is a powerful molecular biology technique used to quantify the amount of a specific DNA sequence in a sample. It builds upon the principles of traditional PCR but incorporates fluorescent dyes or probes to measure DNA amplification in real time.

qPCR technology typically involves the following procedure:

  • Denaturation:
    • The thermocycler heats up to roughly 95 °C.
    • The double-stranded DNA separates into single strands.
  • Annealing:
    • The temperature lowers down to 45-65 °C.
    • The single-stranded primers attach to the appropriate ends of the target sequence.
  • Extension:
    • The temperature is raised to around 68-72°C.
    • DNA polymerase synthesizes a new DNA strand using the primers as templates.
  • Cycling:
    • The thermal cycles are repeated multiple times (usually 20-40 cycles).
    • Each cycle doubles the amount of DNA.
  • Quantification:
    • The qPCR instrument with high-sensitivity spectrometers records the fluorescence emitted at the end of each amplification cycle.


Our qPCR diagnostic products cover a wide range of applications listed in the test menu below.

The qPCR reagents are designed to be used with our special qPCR Instrument. Our qPCR instrument is designed for small-scale testing in research labs and point-of-care testing (POCT), delivering fast and accurate DNA analysis.


Test Menu

Cat. No. Product Name Detection of Package
WI6200 Cellex qSARS-CoV-2/Flu A/Flu B PCR Assay Flu A, Flu B, and Covid-19 virus 48 T/K
WI6201 Cellex qRSV/ADV/hMPV PCR Assay RSV, Adnovirus, and Human metapneumoviruses 48 T/K
WI6202 Cellex qHPIV/RV/EV PCR Assay Human parainfluenza virus, rhinovirus, and enterovirus 48 T/K
WI6203 Cellex qCoV PCR Assays Human common cold Cov 229E, NL63, OC43, and HKU1 48 T/K
WI6204 Cellex qSTD Triple PCR Assays Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis 48 T/K
WI6205 Cellex qMP PCR Tripple Assays Mycoplasma genitalium/Mycoplasma hominis/Human ureaplasma (U. urealyticum & U. parvum) 48 T/K
WI6206 Cellex qGBS PCR Assay Group B streptococcus 48 T/K
WI6207 Cellex qY MD PCR Test Y chromosome microdeletions associated with male infertility 48 T/K
WI6208 Cellex qMTHFR C677T PCR Assay Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, associated with various diseases (vascular, cancers, neurology, diabetes, psoriasis, etc.) 48 T/K
WI6209 Cellex qHPV 24 Dx Assay Detection of all 24 HPV types 48 T/K
WI6210 Cellex qHPV 12 Dx Assay Detection and grouping of 12 High-Risk HPV types including 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 48 T/K
WI6211 Cellex qHPV 11 Dx Assay Detection and grouping of 11 High-Risk HPV types including 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68 48 T/K
WI6212 Cellex qCC Screening Assay Screening of colon cancer 48 T/K
WI6213 Cellex qDEN Multiplex PCR Assay Dengue virus types 1-4; Detect and differentiate Types 1 and 2; detect Types 3 and 4 as a group 48 T/K
WI6214 Cellex qAPOE4 PCR Assay Detection and differentiation of heterozygous and homozygous of APOE4 mutation indicative of Alzheimer’s risk 48 T/K
WI6215 Cellex qSD PCR Assay Sickle cell disease 48 T/K
WI6220 Cellex qEGFR Multiplex PCR Assay EGFR gene mutations of Ex19Del, L858R, T790M, which cause non-small-cell lung cancer 48 T/K
WI6221 Cellex qBRAF V600E PCR Assay Thyroid cancer caused by BRAF V600E mutation 48 T/K
WI6222 Cellex qALK/ROS1/MET PCR Assay ALK/ROS1/MET gene fusions and mutations, which cause lung adenocarcinomas 48 T/K
WI6223 Cellex qKRAS/BRAF/NRAS/PIK3CA PCR Assay Mutations in KRAS/BRAF/NRAS/PIK3CA genes, which cause colorectal cancer 48 T/K
WI6224 Cellex qHER2 PCR Assay HER2 mutation, which causes breast cancer 48 T/K
WI6225 Cellex qJAK2 V617F PCR Assay JAK2 V617F mutation, which causes myeloproliferative cancer 48 T/K